This shows the preparation of the donor material. The extremely thin “Descemet’s Membrane” and the endothelial cells it carries are carefully peeled from the back of the donor cornea. A blue dye is used to make the transparent membrane slightly more visible. The disc of tissue is transferred to a dish to await transplantation. It rolls into a tight scroll because of the elasticity of the membrane.
The disc of tissue, stained again with blue dye is sucked up into a glass pipette. It is then injected into the eye (which has been prepared by removing the equivalent diseased tissue).
In this video, running at 8x real speed, the tissue is unfolded using tiny air bubbles and gentle jets of fluid before pressing into place with a large air bubble for five minutes.